Author Archives: azaleaazelia

  The main principle behind this test is related to the formation of immune complexes between  the antibody and a given antigen. If the concentration of antigen-antibody complex is low, then the immune complexes  remain in suspension as fine particles, … Continue reading →

  IMMUNOLOGICAL ASSAYS Measurements of immunoglobulin The introduction of automated machines to measure immunoglobulins and other proteins has proceeded rapidly in recent decades. Most clinical immunology laboratories rely almost exclusively on these machines, and research labs are also introducing these … Continue reading →

  However, a word of caution is warranted. These hybridoma clones can sometimes partially lose their antibody production so that they are no longer secrete as much antibody as before and may even stop also lose their specificity so that … Continue reading →

  MONOCLONAL ANTIBODIES Over the past two decades, the revolutionary experiments  of Kohler and Milstein have been a major advance in the production of antigen-specific antibodies, In brief, the key to this remarkable advance was the ability to obtain spleen … Continue reading →

  ANTIBODY PRODUCTION POLYCLONAL ANTIBODIES Many mammals have been used to produce antibodies, ranging from the horse, sheep, and goat down to mice and guinea pigs. Often an animals species is selected for antibody production because it will produce less-cross-reactive … Continue reading →

  The various assays can be conveniently divided into two main divisions, some assays are quantitative in that they produce precise result. Many of these assays are automated and can be related to international standards. Qualitative assays are less specific … Continue reading →

  The sensitivity of a test is defined as the number of diseased  individuals that are positive for the test compared with those who are negative. The specifity of a test is the proportion of individuals without a given disease … Continue reading →