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Continuous and discontinuous RNA synthesis in coronaviruses part 23

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Continuous and discontinuous RNA synthesis in coronaviruses part 22

  Coronavirus transcription model Experimental data on transcription in coronaviruses and the related arteriviruses can be integrated into a transcription model that includes three steps. First, gRNA forms transcription initiation precomplexes, bringing into physical proximity the distal TRS-L and TRS-B. … Continue reading

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Continuous and discontinuous RNA synthesis in coronaviruses part 21

  The secondary structure of the active domain and the high-order structure formed by the RNA-RNA interactions could also promote the slowdown and stoppage of the transcription complex at the CS-N, as described for tombusvirus transcription. The sequences and seccondary … Continue reading

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Continuous and discontinuous RNA synthesis in coronaviruses part 20

This interaction could bring into physical proximity the leader sequence, at the genome 5’end, and the TRS-N, which would promote the template switch during synthesis of the negative-strand sgRNAs. This long-distance RNA-RNA interaction provided for the first time experimental support … Continue reading

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Continuous and discontinuous RNA synthesis in coronaviruses part 19

  The amount of sgmRNA N produced is directly proportional to the extent of the complementarity between pE and dE and inversely proportional to the distance between them. This interaction is probably necessary to relocate the active domain, another cis-acting … Continue reading

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Continuous and discontinuous RNA synthesis in coronaviruses part 18

  In TGEV, two intragenomic, long-distance RNA-RNA interactions have been described to regulate the transcription of sgmRNA [coding for the nucleocapsid protein (N protein)], which is the most abundant sgmRNA during viral infection despite its low ΔG value for TRS-L-TRS-B … Continue reading

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Continuous and discontinuous RNA synthesis in coronaviruses part 17

  The coronavirus discontinuous transcription process implies a premature termination during the synthesis of the negative-strand RNAs and a template switch of the nascent negative strand RNA to the leader. This switch requires long-distance RNA-RNA interactions, probably assisted by RNA-protein … Continue reading

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Continuous and discontinuous RNA synthesis in coronaviruses part 16

  These observations provided experimental evidence for the selection of the TRS-L during the template switch, excluding other genome TRS-Bs that contain the CS. Only the CS-L, located in a sequence context with optimal secondary structure and stability for template … Continue reading

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Continuous and discontinuous RNA synthesis in coronaviruses part 15

  Coronavirus transcription resembles high-frequency, similarity-assisted copy-choice RNA recombination, requiring sequence identity between donor and acceptor RNAs and hairpin structures present in the acceptor RNA, in which the TRS-L would act as an acceptor for the cTRS-B donor sequence. Secondary … Continue reading

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Continuous and discontinuous RNA synthesis in coronaviruses part 14

  By engineering the base-pairing between the CS-L and cCS-B in infectious genomic cDNAs  of coronaviruses and arteriviruses, it was normally demonstrated that the discontinuous step of transcription occurs during the synthesis of the negative-strand RNA, and base-pairing between the … Continue reading

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