Fertilization can be attempted using two common methods:
- Conventional insemination. During conventional insemination, healthy sperm and mature eggs are mixed and incubated overnight.
- Intracytoplasmic sperm injection (ICSI). In ICSI, a single healthy sperm is injected directly into each mature egg. ICSI is often used when semen quality or number is a problem or if fertilization attempts during prior IVF cycles failed.