Architecture of SARS-CoV-2 S glycoprotein trimer

To enable single-particle cryo-EM study of the SARS-CoV-2 S glycoprotein, the investigation designed a prefusion stabilized ectodomain trimer construct with an abrogated furin S1/S2 cleavage, two consecutive proline stabilizing mutations, and a C-terminal foldon trimerization domain.