As expected, SARS-CoV-2 Sfur/mut-MLV harbored uncleaved S upon budding. The observed transduction efficiency of VeroE6 cells were higher for SARS-CoV-2 Sfur/mur-MLV than for SARS-CoV-2 S-MLV, whereas the opposite trend was observed for transduction of hACE2-expressing BHK cells.