Both pseudoviruses entered cells equally well, suggesting that SARS-CoV-2 S-MLV could use African green monkey ACE2 as entry receptor. To confirm these results, entry evaluation into BHK cells and observed that transient transfection with hACE2 rendered them susceptible to transduction with SARS-CoV-2 S-MLV. These results demonstrate hACE2 is a functional receptor for SARS-CoV-2.