The SL-CoVs from Rhinoluphus sinicus, including Rs3367, however, had a single ORF8 with only 32-33% amino acid identities to that of civet SARS-CoV. In contrast, the ORF8 of two novel SL-CoV strains recently reported in Yunnan from another rhinolopid species, Rhinolophus ferrumequinum, exhibited exceptionally high (81,3%) amino acid identity to civet SARS-CoV SZ3. This is consistent with isolate Rf1, a SL-CoV reported earlier from R. ferrumequinum in Hubei Province, of which the ORF8 shared 80,4% amino acid identity to SZ3.