HLA antibody testing part 19

HLA antibodies

 

Other reagent and/or lot-specific variables

Antigens may be represented in the single antigen beads assay by differing numbers of unique beads, there are, for example, in a current reagent lot, three A2 beads and five DQ2 beads but only one C7 bead. Subsaturating antibody may be diluted across multiple beads in a multiplexed assay more so than if only a single bead was present, thereby lowering  mean fluorescence intensity  on any single bead of interest.  For higher antibody levels,  maximum total bead mean fluorescence intensity may be higher  for those antigens with multiple beads. There is no validated methods of combining mean fluorescence intensity across multiple beads, however summing  is frequently  reported in the literature.

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