HLA antibody testing part 13

HLA antibodies

 

Nonspecific serum components may bind to the bead in addition to antibodies of interest

Non specific binding of non-HLA antibodies to beads  (for example, in the presence of drugs, such as intravenous Ig, inflammation, or infection) may increase background mean fluorescence intensity of both control beads and beads of interest; isolated mean fluorescence intensity of a target  bead may be misleadingly high. Control values may provide clues to this issue but are rarely routinely provided in clinical laboratory reports or research studies.

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