HLA antibody testing part 10

HLA antibodies



Defining a positive result

The numeric output of Luminex single antigen beads is a trimmed the mean fluorescence intensity along with other factors in determining a positive result: laboratories validate their single antigen beads assays with known negative and positive sera, establish a working mean fluorescence  intensity threshold for antibody detection, and correlate this threshold with positive flow-cytometry cross-matching results as an important laboratory-specific standardization process within a program. Mean fluorescence intensity thresholds may be modified on the basis of patient history, control values, different HLA loci, recipient HLA typing, and consideration of epitope/antigen groups. As such, a strict mean fluorescence intensity level above which clinically relevant  antibodies are  consistently identified is challenging to define.


About azaleaazelia

A nice person... :)
This entry was posted in Tak Berkategori. Bookmark the permalink.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s