HLA antibody testing part 7

HLA antibodies

 

To perform solid-phase assays, recipient serum is incubated with purified HLA antigens presented on a solid-phase platform (commonly microparticle beads). A fluorescent-conjugated anti-human IgG is added, which binds  to and detects  HLA antibody on its antigen target when the beads are analyzed  on a flow cytometer or Luminex platform. The latter platform generates  a semiquantitative output  for each bead  of mean fluorescence intensity, which is compared with negative control  mean fluorescence intensity to determine if HLA antibody is present, It has proven difficult to align  mean fluorescence intensity  measurements within and between laboratories notwithstanding the increased reporting of this metric in published research.

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