HLA antibody testing part 4

HLA antibodies

 

CELL-BASED ASSAYS: DETECTING HLA ANTIBODIES BEFORE SOLID-PHASE ASSAYS

Panel-reactive antibody testing, in general, estimates the percentage of potential donors to whom a recipient has HLA antibodies and approximates the risks of positive crossmatch. Early panel-reactive antibody assays used panels of 25-60 real donor cells selected to represent the common HLA phenotype distribution of the potential deceased donor population, which were then tested for complement dependent cytotoxicity with recipients sera. Results were subject to change with different donor cells in the panel, were less sensitive for rare HLA antigens, and detected only higher titer antibodies. Although still used in conjunction with other assays, cell panels are no longer used in isolation  for panel-reactive antibody determination. The donor specific  complement-dependent cytotoxicity crossmatch assays detects high-titer donor-specific HLA antibodies required to bind complement for demonstration of antibody presence, whereas lower titer donor-specific HLA antibodies may be detected by flow cytometry cross-matching, with a positive result requiring only antibody binding and not complement activation.

Advertisements

About azaleaazelia

A nice person... :)
This entry was posted in Tak Berkategori. Bookmark the permalink.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s